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Event Type
Research Presentation
Academic Department
Chemistry
Location
Dana Science Building, 2nd floor
Start Date
14-4-2023 1:30 PM
End Date
14-4-2023 3:00 PM
Description
With cysteine-containing proteins playing an important role in most biological processes, the detection and imaging of Cysteine proteins have been of great interest. Here, we present the synthesis and evaluation of two maleimide probes, Rhodamine and Biotin, designed for the detection of Cys-rich proteins. In this design, we postulate that maleimide protein’s alkene substituent would react with the thiol group of the Cysteine to form a covalent bond at a certain pH in the presence of a reducing agent. From this bond, we propose that we would be able to detect Cys-rich proteins and qualitatively and quantitatively analyze the Cysteine concentrations. This methodology provides a way to identify the specific Cys-rich proteins to further explore their roles and activities in related diseases.
Synthesis and evaluation of rhodamine- and biotin- probes for detection of cysteine-containing proteins
Dana Science Building, 2nd floor
With cysteine-containing proteins playing an important role in most biological processes, the detection and imaging of Cysteine proteins have been of great interest. Here, we present the synthesis and evaluation of two maleimide probes, Rhodamine and Biotin, designed for the detection of Cys-rich proteins. In this design, we postulate that maleimide protein’s alkene substituent would react with the thiol group of the Cysteine to form a covalent bond at a certain pH in the presence of a reducing agent. From this bond, we propose that we would be able to detect Cys-rich proteins and qualitatively and quantitatively analyze the Cysteine concentrations. This methodology provides a way to identify the specific Cys-rich proteins to further explore their roles and activities in related diseases.
Comments
Under the direction of Dr. Son Hong Nguyen